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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two more splice variants had been described but in depth nucleotide sequence information and facts just isn't still accessible.
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide usually has the epitope identified because of the antibody.
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This guideline outlines further possible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guidebook outlines further attainable optimization of cellular and immunoassay parameters to read more be sure the best possible benefits are...
Antibodies bound to the blocking peptide not bind into the epitope within the focus on protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Target Data Cyclin E belongs to your remarkably conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which add to the temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and it is degraded as cells development by way of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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